ACELL July 46/1
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چکیده
Lindqvist, Anders, Ina Nordström, Ulf Malmqvist, Patrik Nordenfelt, and Per Hellstrand. Long-term effects of Ca21 on structure and contractility of vascular smooth muscle. Am. J. Physiol. 277 (Cell Physiol. 46): C64–C73, 1999.—Culture of dispersed smooth muscle cells is known to cause rapid modulation from the contractile to the synthetic cellular phenotype. However, organ culture of smooth muscle tissue, with maintained extracellular matrix and cell-cell contacts, may facilitate maintenance of the contractile phenotype. To test the influence of culture conditions, structural, functional, and biochemical properties of rat tail arterial rings were investigated after culture. Rings were cultured for 4 days in the absence and presence of 10% FCS and then mounted for physiological experiments. Intracellular Ca21 concentration ([Ca]i) after stimulation with norepinephrine was similar in rings cultured with and without FCS, whereas force development after FCS was decreased by .50%. The difference persisted after permeabilization with b-escin. These effects were associated with the presence of vasoconstrictors in FCS and were dissociated from its growth-stimulatory action. FCS treatment increased lactate production but did not affect ATP, ADP, or AMP contents. The contents of actin and myosin were decreased by culture but similar for all culture conditions. There was no effect of FCS on calponin contents or myosin SM1/SM2 isoform composition, nor was there any appearance of nonmuscle myosin. FCS-stimulated rings showed evidence of cell degeneration not found after culture without FCS or with FCS 1 verapamil (1 μM) to lower [Ca]i. The decreased force-generating ability after culture with FCS is thus associated with increased [Ca]i during culture and not primarily caused by growth-associated modulation of cells from the contractile to the synthetic phenotype.
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ACELL August 46/2
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متن کاملACELL November 46/5
ESTHER TITOS,1 NAN CHIANG,2 CHARLES N. SERHAN,2 MARIO ROMANO,3 JOAN GAYA,4 GLORIA PUEYO,5 AND JOAN CLÀRIA1 1DNA Unit and 4Hormonal Laboratory, Institut d’Investigacions Biomèdiques August Pi i Sunyer, Hospital Clı́nic and 5Quı́mica Farmacéutica Bayer (Consumer Care Division), Barcelona 08036, Spain; 2Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Periop...
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تاریخ انتشار 1999